Quoth the Raven
the personal blog of a newly-fledged biomedical informatician, about anatomy, computers, life, or just anything she finds interesting that day
Monday, June 16, 2014
Wednesday, September 30, 2009
Anatomy class: Connective tissue and bones of the skull
Last night in anatomy lab, we reviewed connective tissue, including bones in general, and specifically, the bones of the skull. We were able to get a real skull, rather than a plastic replica, but the real ones are so fragile that some of the more delicate bones (like the sphenoid) have been broken out. Still, it's a good review for the upcoming quiz; you just cannot study anatomy in 2D in the same way.
Monday, September 28, 2009
Isolating bacteria through streak plate technique
My streak plate technique is off to a fairly good start, it seems. The streak plate method is a way of isolating bacteria into manageable colonies through a series of dilutions.
If you orient the Petri dish as a clock face, notice how thick the bacterial streaks are around the 6 o'clock position. That's zone 1, where I dipped a sterile loop into the broth containing Serratia marcescens, a pink- or red-colored bacterium often found in showers, feeding off phosphorus or lipids in soaps and shampoos. Zone 1 in my Petri dish is the growth that resulted directly from the broth.
Zone 2, which is the area at an angle to Zone 1, running from about 5 o'clock to about 3 o'clock, is less overgrown, though still pretty thick. This is due to taking the loop out of Zone 1, flaming it till it's sterile, then streaking out from Zone 1. So the bacteria in Zone 1 are correspondingly diluted over a larger area.
Zone 3, at an angle from Zone 2 from about 3 o'clock to about 12 o'clock is made in a similar way--flame the loop until sterile, and then dilute Zone 2 over a larger space. Finally, Zone 4--the lines coming out orthogonally from Zone 3--were made the same way, and usually by this point, the inoculum (sample) is diluted enough to where the bacteria can grow in individual colonies, rather than an overgrown "lawn" like in Zone 1.
My Zone 4's not perfect; I will continue to work on a lighter touch. But for an initial attempt, I'm pretty pleased with my colonies from it.
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Thursday, September 24, 2009
Unknown rods, methylene blue simple stain
I didn't stain this particular slide tonight, but I did point and shoot. Unknown rods with methylene blue staining to make them visible; not enough information to identify them.
UPDATE: Got the title wrong last night; it's methylene blue, not methyl blue. Two different dyes.
Wednesday, September 23, 2009
My first negative stain
Klebsiella pneumoniae, a Gram-negative rod, implicated in pneumonia and urinary tract infections. Stained 23 September 2009, Bellevue College.
Saturday, August 29, 2009
It's like they're in our house
When it comes right down to it, all of the scientific method can be summed up in LeVar Burton's catchphrase: "But you don't have to take my word for it."
Now, Reading Rainbow is over.
The show's run is ending, [John] Grant [, content director,] explains, because no one — not the station, not PBS, not the Corporation for Public Broadcasting — will put up the several hundred thousand dollars needed to renew the show's broadcast rights.
Grant says the funding crunch is partially to blame, but the decision to end Reading Rainbow can also be traced to a shift in the philosophy of educational television programming. The change started with the Department of Education under the Bush administration, he explains, which wanted to see a much heavier focus on the basic tools of reading — like phonics and spelling.
Grant says that PBS, CPB and the Department of Education put significant funding toward programming that would teach kids how to read — but that's not what Reading Rainbow was trying to do.
"Reading Rainbow taught kids why to read," Grant says. "You know, the love of reading — [the show] encouraged kids to pick up a book and to read."